Purification of transfer RNA (m5U54)-methyltransferase from Escherichia coli. Association with RNA
نویسندگان
چکیده
منابع مشابه
Association of RNA polymerase with transcribed regions in Escherichia coli.
We examine the association of the beta-, alpha-, and sigma(70)-subunits of Escherichia coli RNA polymerase (RNAP) and the NusA elongation factor with transcribed regions in vivo by using chromatin immunoprecipitation. RNAP preferentially associates with the promoter-proximal region of several operons, and this preference is particularly pronounced at the lexA-dinF promoter. When cells are grown...
متن کاملCharacterization of the 23 S ribosomal RNA m5U1939 methyltransferase from Escherichia coli.
An Escherichia coli open reading frame, ygcA, was identified as a putative 23 S ribosomal RNA 5-methyluridine methyltransferase (Gustafsson, C., Reid, R., Greene, P. J., and Santi, D. V. (1996) Nucleic Acids Res. 24, 3756-3762). We have cloned, expressed, and purified the 50-kDa protein encoded by ygcA. The purified enzyme catalyzed the AdoMet-dependent methylation of 23 S rRNA but did not act ...
متن کاملThree adjacent transfer RNA genes in Escherichia coli.
A defective varphi80 transducing phage has been isolated that carries a small section of the E. coli chromosome from the region near argH (77 min). This phage contains three E. coli tRNA genes, termed glyTsu(+) (36), tyrT, and thrT. These genes specify the structures of tRNA(2) (Gly) (su(+) (36)) tRNA(2) (Tyr), and tRNA(3) (Thr), respectively. Induction of a single lysogen bearing this phage br...
متن کاملStudies on genetically altered transfer RNA species in Escherichia coli.
The study of tRNA molecules altered by a mutational event can lead to information in 3 important areas: (a) structure-function relationships in tRNA biochemistry; (ft) the genetics of tRNA; and (c) the physiological role of tRNA-isoaccepting species. For example, the elucidation of the structural alteration in a mutant tRNA species which reacts appreciably more slowly than normal tRNA in the en...
متن کاملSynthesis of stable RNA in stringent Escherichia coli cells in the absence of charged transfer RNA.
It has been possible to demonstrate the complete absence of either charged tRNA(Glu) or charged tRNA(Val) at 42 degrees by the use of two stringent strains of E. coli, one temperature-sensitive for glutamyl-tRNA synthetase and the other temperature-sensitive for valyl-tRNA synthetase. In both strains, stable RNA synthesis ceases, and guanosine tetraphosphate accumulates upon incubation at the n...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1988
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1988.tb14396.x